Current Pooled Library Projects

Bi-Specific Synthetic Lethality (SL) DDR shRNA Libraries

A promising direction in the development of anti-cancer drugs with high specificity and low toxicity is the possibility of inhibiting the functions of synthetically lethal (SL) (synergistic) DNA Damage and Repair (DDR) drug targets that are specifically modulated in cancer development. Under NIH SBIR contract HHSN261201200065C, we developed the technology for the construction of novel bi-specific pooled lentiviral shRNA libraries designed for the combinatorial knockdown of any two targets in a set of rationally selected genes. Furthermore, we successfully demonstrated the application of a set of bi-specific SL DDR shRNA libraries to detect SL binary interactions between 360 DDR genes in a panel of cancer cell lines. The 360 DDR Relational Database with annotated gene information, reference SL gene interactions revealed in RNAi combinatorial screens, and predicted signaling pathway models are available to academic and non-profit researchers at no charge. Please visit the Bi-Specific SL DDR shRNA Library Project Summary page for more information.

DECIPHER Project Pooled shRNA Libraries

DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. They are made to cover nearly the entire annotated human gene set and most annotated mouse gene set. Lentiviral shRNA libraries were chosen over synthetic siRNA collections for their advantages in cancer research, specifically for the ability to efficiently transduce a range of cell types, including non-dividing cells, and for the highly effective long-term gene silencing. The pooled format screens follow a simple protocol and do not require access to expensive high-throughput arrayed screen equipment and infrastructure, putting large-scale pooled shRNA knockdown screens within reach of any researcher. Please visit the Cellecta website for additional information on the DECIPHER shRNA Libraries.